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KMID : 0545119910010030169
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Journal of Microbiology and Biotechnology
1991 Volume.1 No. 3 p.169 ~ p.175
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Plasmid Linkage of Bacteriocin Production and Sucrose Fermentation Phenotypes in Pediococcus acidilactici M
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KIM, WANG-JUNE
HA, DUK-MO/RAY, BIBEK
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Abstract
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Pediococcus acidilactici strain M produced a bacteriocin which was proteinaceous, heat stable, and exhibited antimicrobial activity against lactic acid bacteria, variety of food spoilage and pathogenic bacteria. The antimicrobial activity was not caused by H_2O_2 and organic acid, and was remained between pHs of 4.0 to 9. Molecular weight of crude bacteriocin was approximately 2,500. Phenotypic assignment after plasmid cruing experiment demonstrated that a 53.7 kilobase (kb) plasmid, designated as pSUC53, was responsible for the sucrose fermentation phenotype (Suc^+) and a 11. 1 kb plasmid, designated as pBAC11, was associated with bacteriocin production phenotype (Bac^+). Neither of the two plasmids were linked to antibiotic resistance.
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